EGFR Mutation Test Kit (RUO)

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ADPS™ EGFR Mutation Test Kit(RUO)

Detects 44 somatic mutations in the EGFR gene accurately and quickly with a detection sensitivity of 0.01%.
Outstanding limit of detection (LoD) provides precise information on EGFR mutations which can improve cancer research related to EGFR mutations.
Both plasma and FFPE samples can be used.

This kit is for Research Use Only (RUO).
Not for use in diagnostic purposes.

FAM	L858R mutant (copies)	gDNA (copies)
Red	30,000	0
Orange	3,000	27,000
Green	300	29,700
Blue	30	29,970
Violet	3	29,997
Black	0	30,000

FAM

L858R mutant
(copies)

gDNA
(copies)

Red

30,000

Orange

3,000

27,000

Green

300

29,700

Blue

29,970

Violet

29,997

Black

30,000

Exon	Mutation	EGFR Nucleic Acid Sequence (COSMIC ID)
Exon 18	G719X	2155G>A (6252), 2155G>T (6253), 2156G>C (6239)
Exon 19	Ex19Del	2240_2251del12 (6210) , 2239_2247del9 (6218) , 2238_2255del18 (6220) , 2235_2249del15 (6223) , 2236_2250del15 (6225) , 2239_2253del15 (6254) , 2239_2256del18 (6255) , 2237_2254del18 (12367) , 2240_2254del15 (12369) , 2240_2257del18 (12370) , 2239_2248TTAAGAGAAG>C (12382) , 2239_2251>C (12383) , 2237_2255>T (12384) , 2235_2255>AAT (12385) , 2237_2252>T (12386) , 2239_2258>CA (12387) , 2239_2256>CAA (12403) , 2237_2253>TTGCT (12416) , 2238_2252>GCA (12419) , 2238_2248>GC (12422) , 2237_2251del15 (12678) , 2236_2253del18 (12728) , 2235_2248>AATTC (13550) , 2235_2252>AAT (13551) , 2235_2251>AATTC (13552) , 2253_2276del24 (13556) , 2237_2257>TCT (18427) , 2238_2252del15 (23571) , 2233_2247del15 (26038) , 2232_2249del18 (221565) , 2234_2248del15 (1190791)
Exon 20	S768I	2303G>T (6241)
T790M	2369C>T (6240)
Ex20Ins	2307_2308ins9GCCAGCGTG (12376) , 2319_2320insCAC (12377) , 2310_2311insGGT (12378) , 2311_2312ins9GCGTGGACA (13428) , 2309_2310AC>CCAGCGTGGAT (13558)
Exon 21	L858R	2573T>G (6224) , 2573_2574TG>GT (12429)
L861Q	2582T>A (6213)

Exon

Mutation

EGFR Nucleic Acid Sequence (COSMIC ID)

Exon 18

G719X

2155G>A (6252), 2155G>T (6253), 2156G>C (6239)

Exon 19

Ex19Del

2240_2251del12 (6210) , 2239_2247del9 (6218) , 2238_2255del18 (6220) , 2235_2249del15 (6223) , 2236_2250del15 (6225) , 2239_2253del15 (6254) , 2239_2256del18 (6255) , 2237_2254del18 (12367) , 2240_2254del15 (12369) , 2240_2257del18 (12370) , 2239_2248TTAAGAGAAG>C (12382) , 2239_2251>C (12383) , 2237_2255>T (12384) , 2235_2255>AAT (12385) , 2237_2252>T (12386) , 2239_2258>CA (12387) , 2239_2256>CAA (12403) , 2237_2253>TTGCT (12416) , 2238_2252>GCA (12419) , 2238_2248>GC (12422) , 2237_2251del15 (12678) , 2236_2253del18 (12728) , 2235_2248>AATTC (13550) , 2235_2252>AAT (13551) , 2235_2251>AATTC (13552) , 2253_2276del24 (13556) , 2237_2257>TCT (18427) , 2238_2252del15 (23571) , 2233_2247del15 (26038) , 2232_2249del18 (221565) , 2234_2248del15 (1190791)

Exon 20

S768I

2303G>T (6241)

T790M

2369C>T (6240)

Ex20Ins

2307_2308ins9GCCAGCGTG (12376) , 2319_2320insCAC (12377) , 2310_2311insGGT (12378) , 2311_2312ins9GCGTGGACA (13428) , 2309_2310AC>CCAGCGTGGAT (13558)

Exon 21

L858R

2573T>G (6224) , 2573_2574TG>GT (12429)

L861Q

2582T>A (6213)

Tube	Contents	Quantity
	EGFR Master Mixture 1	240 μL/tube
	EGFR Master Mixture 2	240 μL/tube
	EGFR Master Mixture 3	240 μL/tube
	EGFR Master Mixture 4	240 μL/tube
	ADPS™ Smart DNA Polymerase	60 μL/tube
	EGFR Positive Control	160 μL/tube
	Nuclease-Free Water	1.0 mL/tube

Tube

Contents

Quantity

EGFR Master Mixture 1

240 μL/tube

EGFR Master Mixture 2

240 μL/tube

EGFR Master Mixture 3

240 μL/tube

EGFR Master Mixture 4

240 μL/tube

ADPS™ Smart DNA Polymerase

60 μL/tube

EGFR Positive Control

160 μL/tube

Nuclease-Free Water

1.0 mL/tube

EGFR	Disease	Frequency
NSCLC (Non-small-cell Lung carcinoma)	Caucasian	≤ 10%
Asian	≤ 35%

EGFR

Disease

Frequency

NSCLC (Non-small-cell Lung carcinoma)

Caucasian

≤ 10%

Asian

≤ 35%

Skip GNB

EGFR Mutation Test RUO

ADPS™ EGFR Mutation Test Kit(RUO)

Real-time PCR data sample