BRAF Mutation Test Kit (RUO)

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ADPS™ BRAF Mutation Test Kit(RUO)

Detects 4 somatic mutations in BRAF gene quickly and accurately
with the highest detection sensitivity of 0.0001%.
With outstanding limit of detection (LoD), BRAF V600E1, V600E2,
V600D1 and V600D2 mutations can be precisely analyzed.
Improved results may be expected in related cancer research.
Both plasma and FFPE samples can be used.

This kit is for Research Use Only (RUO).
Not for use in diagnostic procedures.

FAM	V600E mutant (copies)	gDNA (copies)
Red	30,000	2,970,000
Orange	30,00	2,997,000
Green	300	2,999,700
Blue	30	2,999,970
Violet	3	2,999,997
Black	0	3,000,000

FAM

V600E mutant
(copies)

gDNA
(copies)

Red

30,000

2,970,000

Orange

30,00

2,997,000

Green

300

2,999,700

Blue

2,999,970

Violet

2,999,997

Black

3,000,000

Exon	Mutation	BRAF Nucleic Acid Sequence (COSMIC ID)
600	V600E1	1799T>A (476)
V600E2	1799_1800TG>AA (475)
V600D1	1799_1800TG>AT (477)
V600D2	1799_1800TG>AC (308550)

Exon

Mutation

BRAF Nucleic Acid Sequence (COSMIC ID)

600

V600E1

1799T>A (476)

V600E2

1799_1800TG>AA (475)

V600D1

1799_1800TG>AT (477)

V600D2

1799_1800TG>AC (308550)

Tube	Contents	Quantity
	BRAF Master Mixture	300 μL/tube
	ADPS™ Smart DNA polymerase	30 μL/tube
	BRAF Positive Control (3x10⁴ copies / 5 μL)	75 μL/tube
	Nuclease-Free Water	150 μL/tube

Tube

Contents

Quantity

BRAF Master Mixture

300 μL/tube

ADPS™ Smart DNA polymerase

30 μL/tube

BRAF Positive Control
(3x10⁴ copies / 5 μL)

75 μL/tube

Nuclease-Free Water

150 μL/tube

BRAF	Disease	Frequency
Melanoma	≤ 50%
Thyroid cancer	≤ 40%
NSCLC (Non-small-cell Lung carcinoma)	≤ 3%
Colorectal cancer	≤ 10%

BRAF

Disease

Frequency

Melanoma

≤ 50%

Thyroid cancer

≤ 40%

NSCLC (Non-small-cell Lung carcinoma)

≤ 3%

Colorectal cancer

≤ 10%

Skip GNB

BRAF Mutation Test RUO

ADPS™ BRAF Mutation Test Kit(RUO)

Real-time PCR data sample